Root discoloration and shoot symptoms in silver birch after Phytophthora infection in vitro.
Identifieur interne : 000069 ( Main/Exploration ); précédent : 000068; suivant : 000070Root discoloration and shoot symptoms in silver birch after Phytophthora infection in vitro.
Auteurs : L. Hamberg [Finlande] ; A. Poimala [Finlande] ; S. Velmala [Finlande] ; J. Perttunen [Finlande] ; Riina Muilu-M Kel [Finlande] ; R. Siev Nen [Finlande]Source :
- Plant biology (Stuttgart, Germany) [ 1438-8677 ] ; 2020.
Abstract
There are no records of established plant pathogenic Phytophthora species in Finnish forests, but they are likely in the future. Therefore, the effects of Phytophthora inoculations on young, ca. two-month-old silver birch (Betula pendula) seedling roots, and shoots were investigated. Visual inspection of dark discolorations, direct PCR and reisolation, and detailed root morphology analyses were used to evaluate the effects of Phytophthora inoculation on roots. Symptoms in leaves and stems were also recorded. Phytophthora was successfully reisolated from 67% of the surface sterilized roots of inoculated seedlings, but not from the non-inoculated control seedlings. Dark discolorations were found more often in the root segments of inoculated seedlings than in control seedlings. In the Phytophthora treated seedlings, discolored root segments were usually linked and found primarily in the main root or lateral roots attached to it, whereas in the control seedlings a few single discolored root segments were scattered throughout the root systems. The number of root segments was lower in the inoculated than in the control seedlings indicating root loss after Phytophthora inoculation. In the shoots of inoculated birches, leaf and shoot wilting was observed. The appearance of wilting in shoots without visible dark discoloration in the base of stems indicated that symptoms originated from roots inoculated with Phytophthora.
DOI: 10.1111/plb.13198
PubMed: 33073496
Affiliations:
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Hamberg</name><affiliation wicri:level="1"><nlm:affiliation>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Poimala, A" sort="Poimala, A" uniqKey="Poimala A" first="A" last="Poimala">A. Poimala</name><affiliation wicri:level="1"><nlm:affiliation>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Velmala, S" sort="Velmala, S" uniqKey="Velmala S" first="S" last="Velmala">S. 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Siev Nen</name><affiliation wicri:level="1"><nlm:affiliation>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant biology (Stuttgart, Germany)</title><idno type="eISSN">1438-8677</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">There are no records of established plant pathogenic Phytophthora species in Finnish forests, but they are likely in the future. Therefore, the effects of Phytophthora inoculations on young, ca. two-month-old silver birch (Betula pendula) seedling roots, and shoots were investigated. Visual inspection of dark discolorations, direct PCR and reisolation, and detailed root morphology analyses were used to evaluate the effects of Phytophthora inoculation on roots. Symptoms in leaves and stems were also recorded. Phytophthora was successfully reisolated from 67% of the surface sterilized roots of inoculated seedlings, but not from the non-inoculated control seedlings. Dark discolorations were found more often in the root segments of inoculated seedlings than in control seedlings. In the Phytophthora treated seedlings, discolored root segments were usually linked and found primarily in the main root or lateral roots attached to it, whereas in the control seedlings a few single discolored root segments were scattered throughout the root systems. The number of root segments was lower in the inoculated than in the control seedlings indicating root loss after Phytophthora inoculation. In the shoots of inoculated birches, leaf and shoot wilting was observed. The appearance of wilting in shoots without visible dark discoloration in the base of stems indicated that symptoms originated from roots inoculated with Phytophthora.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33073496</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1438-8677</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Oct</Month><Day>19</Day></PubDate></JournalIssue><Title>Plant biology (Stuttgart, Germany)</Title><ISOAbbreviation>Plant Biol (Stuttg)</ISOAbbreviation></Journal><ArticleTitle>Root discoloration and shoot symptoms in silver birch after Phytophthora infection in vitro.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1111/plb.13198</ELocationID><Abstract><AbstractText>There are no records of established plant pathogenic Phytophthora species in Finnish forests, but they are likely in the future. Therefore, the effects of Phytophthora inoculations on young, ca. two-month-old silver birch (Betula pendula) seedling roots, and shoots were investigated. Visual inspection of dark discolorations, direct PCR and reisolation, and detailed root morphology analyses were used to evaluate the effects of Phytophthora inoculation on roots. Symptoms in leaves and stems were also recorded. Phytophthora was successfully reisolated from 67% of the surface sterilized roots of inoculated seedlings, but not from the non-inoculated control seedlings. Dark discolorations were found more often in the root segments of inoculated seedlings than in control seedlings. In the Phytophthora treated seedlings, discolored root segments were usually linked and found primarily in the main root or lateral roots attached to it, whereas in the control seedlings a few single discolored root segments were scattered throughout the root systems. The number of root segments was lower in the inoculated than in the control seedlings indicating root loss after Phytophthora inoculation. In the shoots of inoculated birches, leaf and shoot wilting was observed. The appearance of wilting in shoots without visible dark discoloration in the base of stems indicated that symptoms originated from roots inoculated with Phytophthora.</AbstractText><CopyrightInformation>This article is protected by copyright. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hamberg</LastName><ForeName>L</ForeName><Initials>L</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-0009-7768</Identifier><AffiliationInfo><Affiliation>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Poimala</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Velmala</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Perttunen</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muilu-Mäkelä</LastName><ForeName>Riina</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Natural Resources Institute Finland, Korkeakoulunkatu 7, FI-33720, Tampere, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sievänen</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Natural Resources Institute Finland, Latokartanonkaari 9, FI-00790, Helsinki, Finland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biol (Stuttg)</MedlineTA><NlmUniqueID>101148926</NlmUniqueID><ISSNLinking>1435-8603</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Betula pendula
</Keyword><Keyword MajorTopicYN="N">disease</Keyword><Keyword MajorTopicYN="N">lateral roots</Keyword><Keyword MajorTopicYN="N">main roots</Keyword><Keyword MajorTopicYN="N">root pathogen</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>19</Day><Hour>6</Hour><Minute>4</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33073496</ArticleId><ArticleId IdType="doi">10.1111/plb.13198</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Finlande</li></country></list><tree><country name="Finlande"><noRegion><name sortKey="Hamberg, L" sort="Hamberg, L" uniqKey="Hamberg L" first="L" last="Hamberg">L. Hamberg</name></noRegion><name sortKey="Muilu M Kel, Riina" sort="Muilu M Kel, Riina" uniqKey="Muilu M Kel R" first="Riina" last="Muilu-M Kel">Riina Muilu-M Kel</name><name sortKey="Muilu M Kel, Riina" sort="Muilu M Kel, Riina" uniqKey="Muilu M Kel R" first="Riina" last="Muilu-M Kel">Riina Muilu-M Kel</name><name sortKey="Perttunen, J" sort="Perttunen, J" uniqKey="Perttunen J" first="J" last="Perttunen">J. Perttunen</name><name sortKey="Poimala, A" sort="Poimala, A" uniqKey="Poimala A" first="A" last="Poimala">A. Poimala</name><name sortKey="Siev Nen, R" sort="Siev Nen, R" uniqKey="Siev Nen R" first="R" last="Siev Nen">R. Siev Nen</name><name sortKey="Velmala, S" sort="Velmala, S" uniqKey="Velmala S" first="S" last="Velmala">S. Velmala</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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